Learning Objectives

• Know how the pyruvate dehydrogenase complex is regulated
• Be able to draw the TCA cycle (intermediates, enzymes and their regulators)
• Understand the role of anaplerotic reactions and enzymes in the TCA cycle
• Be able to draw and reproduce the ETC in its entirety
• Know where each of the processes are located within a cell
• Explain the concept of respiratory control and what uncouplers do
• Understand how the TCA cycle links with glucose oxidation and ATP production
• Know how NADH2 and FADH2 is regenerated in the cytoplasm for reuse by glycolysis

Shuttle Pathways

• what is the purpose of these shuttles?
• what are the steps of the malate-aspartate shuttle?
• what are the steps of the glycerol-3-phosphate shuttle?

TCA Cycle

• what are the net products of the TCA cycle?
• what is the site of the reactions of the TCA cycle?
• what is the linking reaction? purpose?
• what are the steps of the TCA cycle?
• what steps of the TCA cycle are regulated?